What is the surface area of a 96 well plate?
96-Well Plates
| Cat.No. | Brand | Growth Area |
|---|---|---|
| 83-3596 | Corning® | 0.32cm2 |
| 83-3997 | Corning® | 0.32cm2 |
| 83-3598 | Corning® | 0.32cm2 |
| 83-3599 | Corning® | 0.32cm2 |
How much media can 96 well plate?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
| Width (mm) | 85.60 | 85.47 |
How many cells do you need for a 96 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 24-well | 142475 | 0.24 x 106 |
| 48-well | 150687 | 0.12 x 106 |
| 96-well | 167008 | 0.04 x 106 |
What is the surface area of a 12 well plate?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Media Volume (ml) | |
|---|---|---|
| 245 mm (square) | 500 | 100-150 |
| Dishes | ||
| 6 well | 9.5 | 1.9-2.0 |
| 12 well | 3.8 | 0.76-1.14 |
How many HEk cells are in a 96 well plate?
The capacity of 96 well plate is 1-1.5 lakh cells grow easily. Accordingly you can set a reaction time and number of cell seeded. As my experience, it will be better to seed 5000-10000 cells/well for 72 hrs for HEk and HepG2.
What is surface area formula?
Surface area is the sum of the areas of all faces (or surfaces) on a 3D shape. A cuboid has 6 rectangular faces. To find the surface area of a cuboid, add the areas of all 6 faces. We can also label the length (l), width (w), and height (h) of the prism and use the formula, SA=2lw+2lh+2hw, to find the surface area.
What volume μl of sample Do you pipette into a 96 microwell plate?
The fastest option for adding sample liquids to a 96 well plate is for sure an instrument that can handle 96 channels at the same time, like the epMotion® 96. Each of the 96 channels aspirates 10 µL of your sample and dispenses the volume in all wells at the same time.
How do you incubate a 96 well plate?
Incubate the plate on ice for 30 minutes. Thoroughly mix the contents of each well by pipetting up and down 5–6 times. A multi-channel pipette is desirable for this application. This procedure causes the cells to lyse.
How do you transfer cells to a 96 well plate?
Cell doubling time is an important factor to be considered when adjusting cell density at the beginning of an experiment. Plate 200 µL of cell culture (i.e., 10,000–50,000 cells) into the wells of the sterile 96-well cell culture plate. Incubate the cells for 18 hours at 37°C. Stimulate the cells as desired.
What is a 24 well plate?
Offering 24 high volume (3.1mL) wells in the same convenient footprint of a standard 96-well plate, the Krystal 24 provides a large surface area per well, enabling efficient cell growth.